In the course of a public appearance to Lichfield Cathedral on Monday, King Charles was shouted at by a protester who questioned him about his brother's links with disgraced financier Jeffrey Epstein.
The individual inquired how long the King had been aware about his brother's relationship with Epstein, and also questioned whether he had instructed the police to "cover up" for Andrew.
Amid the heckling, there were crowds of supporters and applause for the King, who was in Staffordshire for an ceremony.
The Duke of York's links with Jeffrey Epstein have been under renewed focus in the past few weeks. The prince, who relinquished his titles earlier this month, has repeatedly denied any wrongdoing.
"For how long have you known about Andrew and Jeffrey Epstein? Have you asked the police to conceal for Andrew?"
The King did not respond but carried on greeting supporters, some of whom chanted "God Save The King". One fan can be overheard asking him for a hug.
A female bystander outside the venue was also heard telling the protester to "leave" and to "be quiet".
The scandal over the Duke of York's links to Epstein has been brought back into focus by misconduct allegations repeated in Ms. Giuffre's recently released book.
In the publication, the accuser says she had sex with the prince on three separate occasions, including once with Epstein and "eight other young girls"
Prince Andrew, who reached a financial settlement with Ms Giuffre in 2022, has repeatedly denied any misconduct. In a previous interview, he told a news program he did not remember meeting her "ever", and that they "did not have any sort of intimate relations."
But in recent times, fresh questions have been asked about how he is able to fund his lifestyle despite not being a active member of the royal family.
The revelation that he has only ever paid a nominal fee on his Royal Lodge has heightened the scrutiny over his living arrangements - even though he made significant contributions initially, including for improvements.
The political party said he should be summoned before members of parliament to give evidence about his lease of his lodge.
But last Thursday, the authorities refused to give parliamentarians time to discuss the prince's titles or his home, despite growing pressure for investigation.
The royal household has not issued a statement on whether Prince Andrew might move out of his home - or where he might reside.
Holistic wellness coach and mindfulness practitioner dedicated to helping others achieve inner harmony.